Calcium phosphate (CaP) polymorphs are non-toxic and biocompatible, making them intriguing for a variety of applications, including hard tissue regeneration, medicine delivery, and vaccine development.Investigating the mechanism of calcium phosphate nucleation and development is critical for understanding disorders caused by pathological mineralization and developing biomimetic materials with suitable properties.The current CaP in situ/batch biomineralization process follows the crystal-formation route of pre-nucleation cluster aggregation of particles larger than 500 microns.However, due to their large size, current synthesis methods may have limited efficiency, scalability, and particle uniformity.The development of new technologies for producing nano- and microparticles in biomedicine is interesting for various reasons.
In this context, a microfluidic-based biomineralization technology is developed, with distinct microfluidic parameters altered, and the chip architecture allows for fine manipulation of the reaction parameters.The obtained reaction conditions yielded a 20-100-micron crystal.
Furthermore, this approach enables the straightforward encapsulation of two different model dyes: methylene blue (MB) and rose Bengal (RB).The proposed microfluidic-aided synthesis technique provides a viable route for future study in particle production and medicine delivery systems..
